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CKTM Medium

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CKTM medium is a semi-selective medium, which is used in combination with modified TMB medium (T5126) or MXV medium (M5131) to detect Xanthomonas campestris pv. vesicatoria (Xcv) in seeds of pepper and tomato. Xcv colonies on plates containing CKTM media are yellow, mucoid, mounded and round. Seed Health Testing: Crop: Pepper (Capsicum annuum) Tomato (lycopersicon lycopersicum) Disease: Bacterial spot Pathogen: Xanthomonas campestris pv. Vesicatoria. Composition of Media: Soya Peptone - 2.0 g/l, Tryptone - 2.0 g/l , Glucose anhydrous - 1.0 g/l , L-glutamine - 6.0 g/l, L-histidine - 1.0 g/l, Di-ammonium hydrogen phosphate ((NH4)2HPO4) - 0.8 g/l, Potassium dihydrogen phosphate (KH2PO4) - 1.0 g/l, Magnesium sulfate anhydrous (MgSO4) - 0.2 g/l, Agar - 15.0 g/l. Method: Dissolve 29.0 grams of the ingredients in distilled water and adjust volume to 900 ml. Dissolve 10 ml of Tween 80 in distilled water and adjust volume to 100 ml. Autoclave the solutions separately (121 ° C, 15 psi for 15 minutes). Prepare sterile antibiotic solutions and add the following amounts per liter medium: 65 mg cephalexin monohydrate (C0110), 12 mg 5fluorouracil (F0123), 0.4 mg tobramycin sulphate (T0153), 100 mg cycloheximide (C0176), 100 mg bacitricin (B0106),10 mg neomycin sulphate (M0135). Allow medium to cool down to ca. 45 ° C - 50 ° C, mix the solutions and add antibiotics. Mix gently to avoid air bubbles and pour plates (15-20 ml per 9.0 cm plate).

Manufacturer: Duchefa Biochemie

SKU: C5140

CAS#

M.F.:C16

M.W.:


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