Sweet potato somatic embryo production is accomplished in two stages. Embryogenic callus is continuously proliferated by subculture on media containing 10 M 2,4-D and 1 M 6-BAP. Increasing the K+ concentration to 40-60 mM doubled the production of embryogenic callus, while the production of non embryogenic callus was reduced by 40%.The development of embryos, triggered by the removal of 2,4-D and 6-BAP, was enhanced by decreasing ammonium (NH4+) from 20 to 10 mM.

Manufacturer: Duchefa Biochemie

SKU: C0228

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