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KBZ Medium

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Product Description

Bacterial speck of tomatoes is caused by the bacterium Pseudomonas syringae pv. tomato (Pst). The bacterium can be introduced by the use of Pst-contaminated seeds. Therefore, detection of Pst in seeds of tomato is common. For the detection of Pst, seeds are first soaked in buffer. Then a stomacher is used for the release of bacteria from the seeds. The bacteria are concentrated by centrifugation. Then dilution plating on two semi-selective media KBZ and KBBC is performed. Suspected colonies are transferred to KB and finally identified by PCR or a pathogenicity assay. Pst forms small, flat and pink-colored colonies on KBZ after ca. 5 days.

Manufacturer: Duchefa Biochemie

SKU: K5129




Link to MSDS




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