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PTSA Medium

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Product Description

PTSA (Peptone Tyrosine Sodium chloride Agar) is a semi-selective medium for the detection of Xanthomonas axonopodis pv. phaseoli in bean seed. The medium is not very selective in comparison with mXCP1, but especially colonies from the var. fuscans are easily recognized on this medium because of their excessive production of visible brown pigment. The non-fuscans isolates of Xap grow well on PTSA medium but their recognition is much more difficult due to the lack of pigment production. For relatively clean seed lots, PTSA medium is useful, but for saprophyte-rich samples mXCP1 is much more suitable. Xap is detected by dilution plating of bacterial extract from seeds on PTSA. Then suspected colonies from PTSA should be transferred to YDC. Finally, the identity of suspected isolates is confirmed by a pathogenicity test or PCR.

Manufacturer: Duchefa Biochemie

SKU: P5135




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