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SCM Medium

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Product Description

Bacterial canker is the most important bacterial disease of tomato. The causal organism is Clavibacter michiganensis subsp. michiganensis (Cmm) and this bacterium can be introduced by contaminated seeds. For the detection of Cmm, tomato seeds are first soaked in buffer. Then a stomacher is used for the release of bacteria from the seeds. After the concentration of the bacteria, dilution plating on two semi-selective media is performed. SCM medium is such a semi-selective media. Actually, there are several modifications in use concerning the used carbon source, LiCl and the addition of antibiotics. This medium is used in combination with D2ANX medium (D5128). After dilution plating suspected isolates are transferred to YDC. Finally the identity of suspected isolates is determined by a pathogenicity test or PCR.

Manufacturer: Duchefa Biochemie

SKU: S5127




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