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X-Gal

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Product Description

X-Gal is a chromogenic substrate of B-galactosidase. X-Gal is used in conjunction with Isopropyl-b-D-1-thiogalactoside (IPTG) (I1401) for the detection of B-galactosidase activity in bacterial colonies in a colorimetric assay in order to distinguish recombinants (white) from non-recombinants (blue). X-gal is cleaved at the B1-4 bond between galactose and the 5-Bromo4-chloro-3-indolyl part of X-Gal by B-galactosidase via hydrolysis. The enzymatic cleavage of X-Gal results in the production of a water insoluble blue dichloro-dibromo-indigo precipitate. In cloning strategies with vectors like Lambda-11, M 13m p18 and 19, pUC18 and 19, pUR222 the E. coli lacZ gene is transformed to lac cells. After transformation, the cells show B-galactosidase activity in the presence of IPTG and X-Gal containing media. The insertion of a DNA fragment into the cloning sites of the lacZ gene results in the disruption of B-galactosidase activity leading to the appearance of white colonies on X-Gal and IPTG containing media. Non recombinant cells produce a blue indigo dye on these media. Should be stored dry at 2-8 ° C or below. Allow to warm to room temperature before opening. Protect from light and moisture. Is soluble in DMSO and DMF.

Manufacturer: Duchefa Biochemie

SKU: X1402

CAS# 7240-90-6

M.F.:C14H15BrClNO6

M.W.:408.6

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