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X-GlcA cyclohexylammonium salt

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Product Description

X-GlcA, 5-Bromo-4-chloro-3-indolyl-B-D-gluchronic acid is a substrate for B-D-glucuronidase (GUS) encoded by the gusA gene. The substrate is used as a qualitative histochemical marker of specific GUS expressions in cells and tissue. X-GlcA is cleaved by GUS at the B1 glucuronic bond between glucuronic acid and the 5-Bromo-4-chloro-3-indolyl part of X-GlcA via hydrolysis. The enzymatic cleavage of X-GlcA results in the precipitation of a water insoluble blue dichloro-dibromo-indigo precipitate. Color formation requires three separate reactions. After enzymatic turnover, the released indoxyl derivative dimerises and is subsequently oxidized to the final indigo dye. Should be stored dry at 2-8 ° C or below. Allow to warm to room temperature before opening. Protect from light and moisture. Is soluble in DMSO and DMF. 5 g and 10 g sizes ship as 5x1g and 10x1g packages.

Manufacturer: Duchefa Biochemie
SKU: X1405

CAS# 114162-64-0

M.F.: C14H13BrClNO7.C6H13N

M.W.:521.8

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