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mCS20ABN Medium

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CS20ABN has been developed by Chang et al. to isolate Xanthomonas campestris pv. campestris (Xcc) from crucifer seeds. The original medium recipe allowed the quick isolation of most isolates of Xcc. However, the recovery of some isolates of Xcc was poor due to pH-dependent sensitivity to neomycin. In the modified version, the pH is lowered to 6.5 by the addition of extra potassium dihydrogen phosphate. This modification improved the recovery of some neomycinsensitive isolates considerably. Contaminated seed lots can be detected by dilution plating of the bacterial extract on mCS20ABN and mFS. Suspected isolates are then transferred to YDC. Finally, the identity of the suspected isolates can be determined by a pathogenicity test using brassica seedlings. The colonies of Xcc and Xanthomonas campestris pv. armoraciae are yellow, mucoid and surrounded by a zone of starch hydrolysis. Seed Health Testing: Crop: Brassica. Disease: Black rot and bacterial leaf spot. Pathogen: Xanthomonas campestris pv. Campestris and Xanthomonas campestris pv. Armoraciae. Composition of Media: Agar - 18.0 g/l, Soluble starch - 25.0 g/l, Soya Peptone - 2.0 g/l, Tryptone - 2.0 g/l, Potassium dihydrogen phosphate (KH2PO4) - 2.8 g/l, Di-ammonium hydrogen phosphate ((NH4)2HPO4) 0.8 g/l, Magnesium sulphate anhydrous (MgSO4 anhydrous) - 0.1952 g/l, L-glutamine - 6.0 g/l, L-histidine - 1.0 g/l, Glucose monohydrate - 1.0 g/l. Method: Dissolve 58.8 grams of ingredients in 900 ml distilled water. Adjust pH to 6.5 and adjust volume to 1000 ml. pH should be 6.5 and not higher! Autoclave the solution (121 ° C, 15 psi, 15 minutes). Prepare sterile antibiotic solutions and add the following amounts per liter medium: 35 mg nystatin (N0138), 40 mg neomycin (M0135), 100 mg bacitracin (B0106). Allow medium to cool down to ca. 45 ° C - 50 ° C and add antibiotics. Mix gently to avoid air bubbles and pour plates (15-20 ml per 9.0 cm plate). Store plates for 4 days at 4 ° C to improve visibility of starch hydrolysis.

Manufacturer: Duchefa Biochemie

SKU: C5122

CAS# N/A

M.F.:N/A

M.W.:N/A

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