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mXCP1 Medium

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Product Description

The mXCP1 (modified Xanthomonas Campestris pv. Phaseoli) medium is a semi-selective medium for the detection of Xanthomonas axonopodis pv. phaseoli (Xap) in bean seed. Both the fuscans and non-fuscans type of Xap grow on mXCP1. However the production of the fuscous pigment only becomes visible after a relatively long incubation. Modification of the medium was necessary because of poor recovery of isolates of the Xap var. fuscans type. Recognition of putative Xap colonies relies on the ability of the Xanthomonas axonopodis pv. phaseoli to hydrolyze starch. The colonies of Xanthomonas axonopodis pv. phaseoli on the mXCP1 plate are surrounded by a clear zone of starch hydrolysis. Detection of Psp and Xap is often performed in combi-assay. Xap is detected by dilution plating of bacterial extract from seeds on mXCP1. Then suspected colonies from mXCP1 should be transferred to YDC. Finally, the identity of suspected isolates is confirmed by a pathogenicity test or PCR. Xap colonies are yellow mucoid, convex and surrounded by a clear zone of starch hydrolysis. Colonies of var. fuscan are distinguished by brown pigmentation.

Manufacturer: Duchefa Biochemie

SKU: X5121



M.W.: N/A

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